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tyramide amplification buffer plus  (Biotium)


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    Structured Review

    Biotium tyramide amplification buffer plus
    Tyramide Amplification Buffer Plus, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tyramide+amplification+buffer+plus/Tyramide+Amplification+Buffer+Plus/bio_rxiv__64898__2026__03__13__711502-124-23-27
    Average 94 stars, based on 30 article reviews
    tyramide amplification buffer plus - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Protocol for detection of glial complement expression in relation to amyloid plaques in mouse brain with combined FISH and IHC
    Article Snippet: Opal 780 Reagent Pack , Akoya Biosciences , Cat#FP1501001KT. .. Tyramide Amplification Buffer Plus , Biotium , Cat#22029-T. ..

    Article Title: OSMRβ-mediated signals on resident fibroblasts restore healing in diabetic skin wounds through promoting angiogenesis and granulation tissue formation.
    Article Snippet: .. After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions. ..

    Article Title: LSD1 serine 166 is a phosphorylation switch for chromatin landscaping, gene activation, and tissue remodeling
    Article Snippet: .. Slides were incubated for 10 min with appropriate fluorophore-conjugated tyramide (CF-dye tyramides, Biotium, CA, USA) in 2 nM final concentration in Tyramide Amplification Buffer Plus (Biotium), supplemented with fresh 0.0015% hydrogen peroxide immediately prior to use. ..

    Article Title: OSMRβ-mediated signals on resident fibroblasts restore healing in diabetic skin wounds through promoting angiogenesis and granulation tissue formation
    Article Snippet: .. After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions. ..

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Article Title: Brain-wide neuronal circuit connectome of human glioblastoma
    Article Snippet: .. Tyramide-based signal amplification was performed by sequential and iterative labeling with HRP-labeled secondary antibodies (Jackson ImmunoResearch, HRP-conjugated donkey anti-goat, 703-035-147; HRP-conjugated donkey anti-rabbit, 711-035-152; all 1:500) for 60 minutes at room temperature, followed by washing 3X for 5 minutes each with TBST, then TSA labeling with either CF568 (Biotium, 10119-198) or CF660R (Biotium, 89493-550) in Tyramide Amplification Buffer Plus (Biotium, 22029) as described by the manufacturer. ..

    Article Title: Protective roles of thrombomodulin in cisplatin-induced nephrotoxicity through the inhibition of oxidative and endoplasmic reticulum stress
    Article Snippet: .. After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer's instructions. ..

    Article Title: Adipose tissue-derived fibroblasts engage in immune-stromal crosstalk during obesity-aggravated atherosclerosis in mice
    Article Snippet: After 30 minutes of baking at 60°C and paraffin removal (Leica BOND Dewax Solution, AR9222, Leica Biosystems), slides were heated for 20 minutes at 100°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2, AR940, Leica Biosystems) and blocked for endogenous peroxidases in 3% hydrogen peroxide for 10 minutes at RT. .. Total of four staining rounds were carried out as follows: Protein blocking by Normal antibody diluent (10 minutes at RT) or Dako REAL antibody diluent (for PI16), primary antibody incubation (20 minutes at RT, Supplementary Table 1), secondary antibody incubation (10 minutes at RT, Supplementary Table 1), tyramide signal amplification by incubating slides with corresponding fluorophore-conjugated tyramide (Supplementary Table 1) for 10 minutes in 2 nM final concentration in Tyramide Amplification Buffer Plus (15129, Biotium), supplemented with fresh 0.0015% hydrogen peroxide. .. Antibodies were removed by heating slides for 10 minutes at 98°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2) and the whole staining cycle was repeated for each marker in the following order: PI16, F4/80, CD4, CD3.

    Incubation:

    Article Title: OSMRβ-mediated signals on resident fibroblasts restore healing in diabetic skin wounds through promoting angiogenesis and granulation tissue formation.
    Article Snippet: .. After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions. ..

    Article Title: LSD1 serine 166 is a phosphorylation switch for chromatin landscaping, gene activation, and tissue remodeling
    Article Snippet: .. Slides were incubated for 10 min with appropriate fluorophore-conjugated tyramide (CF-dye tyramides, Biotium, CA, USA) in 2 nM final concentration in Tyramide Amplification Buffer Plus (Biotium), supplemented with fresh 0.0015% hydrogen peroxide immediately prior to use. ..

    Article Title: OSMRβ-mediated signals on resident fibroblasts restore healing in diabetic skin wounds through promoting angiogenesis and granulation tissue formation
    Article Snippet: .. After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions. ..

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Article Title: Protective roles of thrombomodulin in cisplatin-induced nephrotoxicity through the inhibition of oxidative and endoplasmic reticulum stress
    Article Snippet: .. After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer's instructions. ..

    Article Title: Adipose tissue-derived fibroblasts engage in immune-stromal crosstalk during obesity-aggravated atherosclerosis in mice
    Article Snippet: After 30 minutes of baking at 60°C and paraffin removal (Leica BOND Dewax Solution, AR9222, Leica Biosystems), slides were heated for 20 minutes at 100°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2, AR940, Leica Biosystems) and blocked for endogenous peroxidases in 3% hydrogen peroxide for 10 minutes at RT. .. Total of four staining rounds were carried out as follows: Protein blocking by Normal antibody diluent (10 minutes at RT) or Dako REAL antibody diluent (for PI16), primary antibody incubation (20 minutes at RT, Supplementary Table 1), secondary antibody incubation (10 minutes at RT, Supplementary Table 1), tyramide signal amplification by incubating slides with corresponding fluorophore-conjugated tyramide (Supplementary Table 1) for 10 minutes in 2 nM final concentration in Tyramide Amplification Buffer Plus (15129, Biotium), supplemented with fresh 0.0015% hydrogen peroxide. .. Antibodies were removed by heating slides for 10 minutes at 98°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2) and the whole staining cycle was repeated for each marker in the following order: PI16, F4/80, CD4, CD3.

    Concentration Assay:

    Article Title: LSD1 serine 166 is a phosphorylation switch for chromatin landscaping, gene activation, and tissue remodeling
    Article Snippet: .. Slides were incubated for 10 min with appropriate fluorophore-conjugated tyramide (CF-dye tyramides, Biotium, CA, USA) in 2 nM final concentration in Tyramide Amplification Buffer Plus (Biotium), supplemented with fresh 0.0015% hydrogen peroxide immediately prior to use. ..

    Article Title: Adipose tissue-derived fibroblasts engage in immune-stromal crosstalk during obesity-aggravated atherosclerosis in mice
    Article Snippet: After 30 minutes of baking at 60°C and paraffin removal (Leica BOND Dewax Solution, AR9222, Leica Biosystems), slides were heated for 20 minutes at 100°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2, AR940, Leica Biosystems) and blocked for endogenous peroxidases in 3% hydrogen peroxide for 10 minutes at RT. .. Total of four staining rounds were carried out as follows: Protein blocking by Normal antibody diluent (10 minutes at RT) or Dako REAL antibody diluent (for PI16), primary antibody incubation (20 minutes at RT, Supplementary Table 1), secondary antibody incubation (10 minutes at RT, Supplementary Table 1), tyramide signal amplification by incubating slides with corresponding fluorophore-conjugated tyramide (Supplementary Table 1) for 10 minutes in 2 nM final concentration in Tyramide Amplification Buffer Plus (15129, Biotium), supplemented with fresh 0.0015% hydrogen peroxide. .. Antibodies were removed by heating slides for 10 minutes at 98°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2) and the whole staining cycle was repeated for each marker in the following order: PI16, F4/80, CD4, CD3.

    Immunofluorescence:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Blocking Assay:

    Article Title: OBESITY-INDUCED ENDOTHELIAL FENESTRATION AND CAPILLARY LEAKAGE CONTRIBUTE TO INCREASED PAIN SENSATION
    Article Snippet: Staining was performed using rabbit anti-FOXO1 antibody (Cell Signaling, 2880) and guinea pig anti-K14 antibody (PROGEN, GP-CK14, 1:100). .. To detect FOXO1 immunofluorescence, the skin sections were incubated in the blocking buffer containing anti-rabbit HRP (PerkinElmer, NEF812001EA, 1:250), followed by incubation in Tyramide Amplification Buffer Plus (Biotium, 99832) containing OPAL 520 (Akoya Biosciences, FP1487001KT). .. For K14 detection, the skin sections were incubated in the blocking buffer containing Alexa-568-conjugated secondary antibody (Thermo Fisher Scientific, 1:250).

    Article Title: Adipose tissue-derived fibroblasts engage in immune-stromal crosstalk during obesity-aggravated atherosclerosis in mice
    Article Snippet: After 30 minutes of baking at 60°C and paraffin removal (Leica BOND Dewax Solution, AR9222, Leica Biosystems), slides were heated for 20 minutes at 100°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2, AR940, Leica Biosystems) and blocked for endogenous peroxidases in 3% hydrogen peroxide for 10 minutes at RT. .. Total of four staining rounds were carried out as follows: Protein blocking by Normal antibody diluent (10 minutes at RT) or Dako REAL antibody diluent (for PI16), primary antibody incubation (20 minutes at RT, Supplementary Table 1), secondary antibody incubation (10 minutes at RT, Supplementary Table 1), tyramide signal amplification by incubating slides with corresponding fluorophore-conjugated tyramide (Supplementary Table 1) for 10 minutes in 2 nM final concentration in Tyramide Amplification Buffer Plus (15129, Biotium), supplemented with fresh 0.0015% hydrogen peroxide. .. Antibodies were removed by heating slides for 10 minutes at 98°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2) and the whole staining cycle was repeated for each marker in the following order: PI16, F4/80, CD4, CD3.

    Labeling:

    Article Title: Brain-wide neuronal circuit connectome of human glioblastoma
    Article Snippet: .. Tyramide-based signal amplification was performed by sequential and iterative labeling with HRP-labeled secondary antibodies (Jackson ImmunoResearch, HRP-conjugated donkey anti-goat, 703-035-147; HRP-conjugated donkey anti-rabbit, 711-035-152; all 1:500) for 60 minutes at room temperature, followed by washing 3X for 5 minutes each with TBST, then TSA labeling with either CF568 (Biotium, 10119-198) or CF660R (Biotium, 89493-550) in Tyramide Amplification Buffer Plus (Biotium, 22029) as described by the manufacturer. ..

    Staining:

    Article Title: Adipose tissue-derived fibroblasts engage in immune-stromal crosstalk during obesity-aggravated atherosclerosis in mice
    Article Snippet: After 30 minutes of baking at 60°C and paraffin removal (Leica BOND Dewax Solution, AR9222, Leica Biosystems), slides were heated for 20 minutes at 100°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2, AR940, Leica Biosystems) and blocked for endogenous peroxidases in 3% hydrogen peroxide for 10 minutes at RT. .. Total of four staining rounds were carried out as follows: Protein blocking by Normal antibody diluent (10 minutes at RT) or Dako REAL antibody diluent (for PI16), primary antibody incubation (20 minutes at RT, Supplementary Table 1), secondary antibody incubation (10 minutes at RT, Supplementary Table 1), tyramide signal amplification by incubating slides with corresponding fluorophore-conjugated tyramide (Supplementary Table 1) for 10 minutes in 2 nM final concentration in Tyramide Amplification Buffer Plus (15129, Biotium), supplemented with fresh 0.0015% hydrogen peroxide. .. Antibodies were removed by heating slides for 10 minutes at 98°C in antigen retrieval solution (Leica BOND Epitope Retrieval Solution 2) and the whole staining cycle was repeated for each marker in the following order: PI16, F4/80, CD4, CD3.



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